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8 × 60 k high-density oligonucleotide microarray  (Agilent technologies)


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    Agilent technologies 8 × 60 k high-density oligonucleotide microarray
    Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the <t>microarray</t> was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.
    8 × 60 K High Density Oligonucleotide Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/8+%C3%97+60+k+high-density+oligonucleotide+microarray/pmc10281987-387-5-0?v=Agilent+technologies
    Average 90 stars, based on 1 article reviews
    8 × 60 k high-density oligonucleotide microarray - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell"

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-36354-3

    Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.
    Figure Legend Snippet: Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.

    Techniques Used: Cell Surface Receptor Assay, Microarray

    Differentially expressed genes related to TGFβ and Wnt signaling pathways. ( A ) Cytoscape representation of the genes described in Tables , . The darkest the node color, the highest the fold change (down regulation in green shades, upregulation in red shades; see Tables , for fold change values). ( B ) Expression profile from qRT-PCR analysis of several genes associated to the TGFβ signaling pathway ( smad7-1, smad7-2, dusp6-1, dusp6-2, zeb1b, mmp9 ), to Wnt/β-catenin signaling pathway ( notum1a, frzb ), to both pathways ( bambia, fn1b ), a mesenchymal marker gene ( col1a1a ), and a set of genes related to pluripotency ( nanog, pou2, sox2, c-myca1 and c-myca2 ). Data are presented as fold change (FC) between treated and non-treated (control) cells. Error bars: SEM errors of the FC mean. A total of 5 to 9 paired samples (treated versus control cells) were analyzed per gene. Statistical test was performed using the nonparametric Wilcoxon test comparing the paired normalized expression values between egg extract treated and control cells. *Significant differences (p < 0.05) between treated and control values. Grey bars: upregulated genes; white bars: down regulated genes. Extensions -1 or -2 in the gene name correspond to duplicated gene copies in goldfish. It should be noted that these qPCR data confirm the microarray results indicating a deregulation of both signaling pathways.
    Figure Legend Snippet: Differentially expressed genes related to TGFβ and Wnt signaling pathways. ( A ) Cytoscape representation of the genes described in Tables , . The darkest the node color, the highest the fold change (down regulation in green shades, upregulation in red shades; see Tables , for fold change values). ( B ) Expression profile from qRT-PCR analysis of several genes associated to the TGFβ signaling pathway ( smad7-1, smad7-2, dusp6-1, dusp6-2, zeb1b, mmp9 ), to Wnt/β-catenin signaling pathway ( notum1a, frzb ), to both pathways ( bambia, fn1b ), a mesenchymal marker gene ( col1a1a ), and a set of genes related to pluripotency ( nanog, pou2, sox2, c-myca1 and c-myca2 ). Data are presented as fold change (FC) between treated and non-treated (control) cells. Error bars: SEM errors of the FC mean. A total of 5 to 9 paired samples (treated versus control cells) were analyzed per gene. Statistical test was performed using the nonparametric Wilcoxon test comparing the paired normalized expression values between egg extract treated and control cells. *Significant differences (p < 0.05) between treated and control values. Grey bars: upregulated genes; white bars: down regulated genes. Extensions -1 or -2 in the gene name correspond to duplicated gene copies in goldfish. It should be noted that these qPCR data confirm the microarray results indicating a deregulation of both signaling pathways.

    Techniques Used: Expressing, Quantitative RT-PCR, Marker, Microarray

    Gene Ontology (GO) flow diagram of the terms related to lipid metabolic process ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of genes downregulated in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. ( A ) The black and the dotted lines represent respectively direct and indirect connections between GO terms. ( B ) Dre, Danio rerio prefix of the KEGG identifier. For each GO term and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B figures highlight the disturbance of lipid metabolism after egg extract treatment, and specifically cholesterol and fatty acid biosynthesis.
    Figure Legend Snippet: Gene Ontology (GO) flow diagram of the terms related to lipid metabolic process ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of genes downregulated in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. ( A ) The black and the dotted lines represent respectively direct and indirect connections between GO terms. ( B ) Dre, Danio rerio prefix of the KEGG identifier. For each GO term and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B figures highlight the disturbance of lipid metabolism after egg extract treatment, and specifically cholesterol and fatty acid biosynthesis.

    Techniques Used: Microarray



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    8 × 60 k high-density oligonucleotide microarray  (Agilent technologies)
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    Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the <t>microarray</t> was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.
    8 × 60 K High Density Oligonucleotide Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the <t>microarray</t> was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.
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    Image Search Results


    Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.

    Journal: Scientific Reports

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell

    doi: 10.1038/s41598-023-36354-3

    Figure Lengend Snippet: Gene Ontology (GO) flow diagram of the terms related to cell surface receptor signaling pathway ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of upregulated genes in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. A: The black and the dotted lines represent respectively direct and indirect and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B highlight the disturbance of the TGFβ and Wnt signaling pathways in response to egg extract treatment.

    Article Snippet: Agilent 8 × 60 K high-density oligonucleotide microarray (GEO platform no. GPL32340) was spotted with a set of 52,362 distinct goldfish oligonucleotides.

    Techniques: Cell Surface Receptor Assay, Microarray

    Differentially expressed genes related to TGFβ and Wnt signaling pathways. ( A ) Cytoscape representation of the genes described in Tables , . The darkest the node color, the highest the fold change (down regulation in green shades, upregulation in red shades; see Tables , for fold change values). ( B ) Expression profile from qRT-PCR analysis of several genes associated to the TGFβ signaling pathway ( smad7-1, smad7-2, dusp6-1, dusp6-2, zeb1b, mmp9 ), to Wnt/β-catenin signaling pathway ( notum1a, frzb ), to both pathways ( bambia, fn1b ), a mesenchymal marker gene ( col1a1a ), and a set of genes related to pluripotency ( nanog, pou2, sox2, c-myca1 and c-myca2 ). Data are presented as fold change (FC) between treated and non-treated (control) cells. Error bars: SEM errors of the FC mean. A total of 5 to 9 paired samples (treated versus control cells) were analyzed per gene. Statistical test was performed using the nonparametric Wilcoxon test comparing the paired normalized expression values between egg extract treated and control cells. *Significant differences (p < 0.05) between treated and control values. Grey bars: upregulated genes; white bars: down regulated genes. Extensions -1 or -2 in the gene name correspond to duplicated gene copies in goldfish. It should be noted that these qPCR data confirm the microarray results indicating a deregulation of both signaling pathways.

    Journal: Scientific Reports

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell

    doi: 10.1038/s41598-023-36354-3

    Figure Lengend Snippet: Differentially expressed genes related to TGFβ and Wnt signaling pathways. ( A ) Cytoscape representation of the genes described in Tables , . The darkest the node color, the highest the fold change (down regulation in green shades, upregulation in red shades; see Tables , for fold change values). ( B ) Expression profile from qRT-PCR analysis of several genes associated to the TGFβ signaling pathway ( smad7-1, smad7-2, dusp6-1, dusp6-2, zeb1b, mmp9 ), to Wnt/β-catenin signaling pathway ( notum1a, frzb ), to both pathways ( bambia, fn1b ), a mesenchymal marker gene ( col1a1a ), and a set of genes related to pluripotency ( nanog, pou2, sox2, c-myca1 and c-myca2 ). Data are presented as fold change (FC) between treated and non-treated (control) cells. Error bars: SEM errors of the FC mean. A total of 5 to 9 paired samples (treated versus control cells) were analyzed per gene. Statistical test was performed using the nonparametric Wilcoxon test comparing the paired normalized expression values between egg extract treated and control cells. *Significant differences (p < 0.05) between treated and control values. Grey bars: upregulated genes; white bars: down regulated genes. Extensions -1 or -2 in the gene name correspond to duplicated gene copies in goldfish. It should be noted that these qPCR data confirm the microarray results indicating a deregulation of both signaling pathways.

    Article Snippet: Agilent 8 × 60 K high-density oligonucleotide microarray (GEO platform no. GPL32340) was spotted with a set of 52,362 distinct goldfish oligonucleotides.

    Techniques: Expressing, Quantitative RT-PCR, Marker, Microarray

    Gene Ontology (GO) flow diagram of the terms related to lipid metabolic process ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of genes downregulated in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. ( A ) The black and the dotted lines represent respectively direct and indirect connections between GO terms. ( B ) Dre, Danio rerio prefix of the KEGG identifier. For each GO term and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B figures highlight the disturbance of lipid metabolism after egg extract treatment, and specifically cholesterol and fatty acid biosynthesis.

    Journal: Scientific Reports

    Article Title: TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell

    doi: 10.1038/s41598-023-36354-3

    Figure Lengend Snippet: Gene Ontology (GO) flow diagram of the terms related to lipid metabolic process ( A ) and KEGG pathways ( B ). The analysis was performed on the cluster of genes downregulated in treated cells (fold change > 2) using WebGestalt web tool. The set of genes spotted on the microarray was used as the reference gene list. ( A ) The black and the dotted lines represent respectively direct and indirect connections between GO terms. ( B ) Dre, Danio rerio prefix of the KEGG identifier. For each GO term and KEGG pathway ( www.kegg.jp/kegg/kegg1.html ), p values (P) below 0.05 and false discovery rate (FDR) below 0.05 are indicated. Both A and B figures highlight the disturbance of lipid metabolism after egg extract treatment, and specifically cholesterol and fatty acid biosynthesis.

    Article Snippet: Agilent 8 × 60 K high-density oligonucleotide microarray (GEO platform no. GPL32340) was spotted with a set of 52,362 distinct goldfish oligonucleotides.

    Techniques: Microarray